全部商品分类
下载产品说明书 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询Scientific Data
View LargerRecombinant Mouse VSIG3 Fc Chimera (Catalog # 9367-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED50 for this effect is 1-5 μg/mL.
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9367-VS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Recombinant Mouse VSIG3 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse VSIG3 (Leu23-Gly245) Accession # P0C673 | IEGRMDP | Mouse IgG2a (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Background: VSIG3
VSIG3 (V-set and immunoglobulin domain containing 3) also known as IgSF11 (Immunoglobulin superfamily member 11), is an approximately 50 kDa type I transmembrane adhesion protein of the Ig superfamily. Mature mouse VSIG3 consists of a 218 amino acid (aa) extracellular domain (ECD) containing one V-type Ig-like domain and one Ig-like C2-type domain, a 21 aa transmembrane domain, and a 167 aa cytoplasmic domain. Within the ECD, mouse VSIG3 shares 95% and 98% aa identity with human and rat VSIG3, respectively. VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (1). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (1-3). VSIG3 also localizes to neuronal postsynaptic densities where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (4). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (5). VSIG-3 expression is elevated in colorectal cancers and hepatocellular carcinomas as well as
intestinal-type gastric cancers. Suppression of VSIG-3 by siRNA retarded the growth of gastric cancer cells, suggesting that VSIG-3 is a good candidate for gastric cancer immunotherapy (6).
- Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
- Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
- Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
- Jang, S. et al. (2016) Nat. Neurosci. 19:84.
- Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
- Watanabe T, et al. (2005) Cancer Sci. 96:498.
参考图片
Recombinant Mouse VSIG3 Fc Chimera (Catalog # 9367-VS)inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.The ED50 for this effect is 1-5 μg/mL.