rmTIGIT Fc, CF (1 MG)

rmTIGIT Fc, CF (1 MG)

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品牌: R&D Systems
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7267-TG

FormulationLyophilized from a 0.2 μm filtered solution in PBS.
ReconstitutionReconstitute at 100 μg/mL in PBS.
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Recombinant Mouse TIGIT Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD155/PVR (Catalog # 6909-CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse TIGIT Fc Chimera that produces 50% optimal binding response is 10‑50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TIGIT protein
Mouse TIGIT
(Gly26-Thr143)
Accession # NP_001139797
IEGRMDPMouse IgG2A
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gly26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40.1 kDa (monomer)
SDS-PAGE
50-60 kDa, reducing conditions
背景
别名
T cell immunoreceptor with Ig and ITIM domains,TIGIT,VSIG9,VSTM3,WUCAM,T Cell Immunoreceptor with Ig and ITIM Domains
背景

Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V‑set and transmembrane domain‑containing 3), Vsig9 (V‑set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30‑34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1‑4). Mouse TIGIT cDNA encodes 241 amino acids (aa) including a 26 aa signal sequence, a 115 aa extracellular region with a V‑type Ig-like domain and one potential N‑glycosylation site, a 21 aa transmembrane sequence, and a 79 aa cytoplasmic domain with an ITIM motif (5). A 249 aa variant contains inserted aa after aa 21, 131 and 164 (6). Within the ECD, mouse TIGIT shares only 59‑70% aa sequence identity with human, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 7‑9). It binds to CD155/PVR/Necl‑5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1‑3, 8). Binding of TIGIT by DC induces IL‑10 release and inhibits IL‑12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (7‑9). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 7, 8). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM‑1 (7, 8). Soluble TIGIT is able to compete with DNAM‑1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 9, 10).

References
  1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
  2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
  3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
  4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
  5. NCBI Reference Sequence Accession # NP_001139797.
  6. Swissprot Accession # P86176.
  7. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  8. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
  9. Joller, N. et al. (2011) J. Immunol. 186:1338.
  10. Lozano, E. et al. (2012) J. Immunol. 188: Mar. 16 [Epub ahead of print].
Long Name
T Cell Immunoreceptor with Ig and ITIM Domains
Entrez Gene IDs
201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)
Alternate Names
T cell immunoreceptor with Ig and ITIM domains; TIGIT; VSIG9; VSTM3; WUCAM
制备和贮存
溶解方法
Reconstitute at 100 μg/mL in PBS.
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货号:
7267-TG-01M
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1MG
50ug
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