hCD133 PE MAb (Cl 17 (100 Tests)

hCD133 PE MAb (Cl 17 (100 Tests)

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品牌: R&D Systems
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来源宿主:
Mouse IgG2a
实验应用:
Flow Cytometry
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
AC133 Stargardt disease 4 (autosomal dominant), AC133, CD133 retinal 2, CD133, PROM1, prominin (mous
宿主
Mouse IgG2a
免疫原
NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490
克隆号
170411

Scientific Data

Flow Cytometry Detection of CD133 antibody in NS0 Mouse Cell line Transfected with Human CD133 antibody by Flow Cytometry.View Larger

Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 PE‑conjugated Monoclonal Antibody (Catalog # FAB11331P). View our protocol for Staining Membrane-associated Proteins.

Human CD133 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD133. Stains human CD133 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 170411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Flow Cytometry
背景
别名
AC133 Stargardt disease 4 (autosomal dominant),AC133,CD133 retinal 2,CD133,PROM1,prominin (mouse)-like 1,Prominin 1,PROML1
背景

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells. 

Entrez Gene IDs
8842 (Human); 19126 (Mouse)
Alternate Names
AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1
制备和贮存
保存方式

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

参考图片

Detection of CD133 in NS0 Mouse Cell line Transfected with Human CD133 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human CD133 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human CD133 PE‑conjugated Monoclonal Antibody (Catalog # FAB11331P). View our protocol for Staining Membrane-associated Proteins.

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货号:
FAB11331P-100
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询价
25Tests
100Tests
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