hADORA2A A647 MAb (C (100 Tests)

hADORA2A A647 MAb (C (100 Tests)

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品牌: R&D Systems
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来源宿主:
Mouse IgG2a
实验应用:
Flow Cytometry
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
A2aR, adenosine A2 receptor, Adenosine A2a R, adenosine A2a receptor, Adenosine A2aR, adenosine rece
宿主
Mouse IgG2a
免疫原
NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274
克隆号
599717

Scientific Data

Flow Cytometry Detection of Adenosine A2a R antibody in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP antibody by Flow Cytometry.View Larger

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB94971R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

Human Adenosine A2aR Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adenosine A2a R in HEK293 human cell line transfected with Human Adenosine A2a R and not irrelavant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 599717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Flow Cytometry
背景
别名
A2aR,adenosine A2 receptor,Adenosine A2a R,adenosine A2a receptor,Adenosine A2aR,adenosine receptor A2a,adenosine receptor subtype A2a,ADORA 2,ADORA2,ADORA2A,hA2aR,RDC8,Adenosine A2A Receptor
背景

Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name
Adenosine A2A Receptor
Entrez Gene IDs
135 (Human); 11540 (Mouse); 25369 (Rat)
Alternate Names
A2aR; adenosine A2 receptor; Adenosine A2a R; adenosine A2a receptor; Adenosine A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA 2; ADORA2; ADORA2A; hA2aR; RDC8
制备和贮存
保存方式

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

参考图片

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB94971R). Quadrant markers were set based on control antibody staining (Catalog # IC003R). View our protocol for Staining Membrane-associated Proteins.

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货号:
FAB94971R-100
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询价
100Tests
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