Luminex检测原理

Luminex® Assay 与High Performance分析检测均使用带有颜色编码的聚苯乙烯微球或超顺磁微球,这些微球表面包被了针对目标物的特异性抗体。当这些预包被的微球与待检样本预混孵育时,目标物被微球捕获后,被用生物素标记的特异性检测抗体混合物识别,加入链霉亲和素-PE荧光素底物后发光并进行检测。

试剂盒有两种形式可供选择:聚苯乙烯微球或磁性微球

  • ◇ 聚苯乙烯微球可用于:Luminex 100™,Luminex 200™,或Bio-Rad® Bio-Plex®双激光分析仪。
  • ◇ 磁性微球可用于:Luminex MAGPIX®,Luminex 100™,Luminex 200™,或Bio-Rad® Bio-Plex®分析仪。

Luminex 液相芯片和高性能液相芯片

R&D Systems提供Luminex Assay与 High Performance 2个系列的试剂盒,能够同时对多种复杂样本中的多个指标进行检测和定量。Luminex基于微球设计原理,能够针对每一个指标提供准确、可重复的检测结果,仅需少量样本,成本更低,成功实现短时间内获得比其他检测方法更多的数据。

Luminex High Performance系列

基于微球材料,Luminex High Performance系列预先优化检测指标组合,实现多重检测结果更精准,能够同时检测复杂基质样本的多达22种指标。因子可以按照完整的panel提供,也可以提供自由组合形式。Luminex Performance 系列可以验证样本类型:细胞上清、血清、血浆、尿液、唾液和牛奶。

优势
  • 完整的panel组合:All analytes in a given panel are optimized and tested together to ensure maximum performance for the entire panel
  • 和ELISA媲美的准确性:Kits undergo similar validation testing as the Quantikine® single analyte assays and are correlated with Quantikine ELISAs, when available
  • 自由订货形式选择:Kits consist of base kits and bead sets, which can be ordered individually or as premixed kits
  • 种属:Kits are available for human and rat analytes
  • 省时:All assays can be performed in 2.5-5 hrs
  • 样本上样量:Assays require a small sample volume (<100 µL)
Luminex® High Performance Assays Correlate to Quantikine® ELISAs
  • Cell culture supernate from adipocytes were analyzed using both Luminex® High Performance Assays and Quantikine® ELISAs. Cell culture supernate from pre-adipocytes before and after differentiation were harvested at the time points indicated and frozen. Following collection, samples were analyzed using Human Leptin and Human Total MMP-3 Quantikine® ELISAs (blue bars; Catalog # DLP00 and DMP300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LUB000 and LMP000B, respectively).

  • Cell culture supernate from SW1353 human chondrosarcoma cells were analyzed using both Luminex® High Performance Assays and Quantikine®ELISAs. Cell culture supernate from SW1353 cells were either unstimulated or stimulated with 5 ng/mL IL-1 alpha and 10 ng/mL OSM, harvested 24 hours later, and frozen. Following collection, samples were analyzed using Human CXCL10/IP-10, Human Total MMP-3, and Human Pro-MMP-13 Quantikine® ELISAs (blue bars; Catalog # DIP100, DMP300, and DM1300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LUB000 and LMP000B, respectively).

试剂盒组分:
  • Base Kits(基础试剂盒): Standard cocktail(s), bead diluent, biotin antibody diluent, calibrator diluent(s), Streptavidin-PE, wash buffer concentrate, mixing bottles, filter-bottom or round-bottom microplate, foil plate sealers
  • Bead Sets: Bead Sets: Antibody-coated beads, biotinylated detection antibody cocktail
  • *Base Kits and Bead Sets can be ordered individually or as premixed kits.
查看商品列表 文献欣赏
  • +Human Angiogenesis Panel A

  • +Human Biomarker Panel A

  • +Human Cardiac Panel A

  • +Human Cardiac Panel B

  • +Human Cytokine Panel A

  • +Human Cytokine Panel B

  • +Human High Sensitivity Cytokine Panel A

  • +Human High Sensitivity Cytokine Panel B

  • +Human Kidney Biomarker Panel

  • +Human MMP Panel

  • +Human Obesity Panel

  • +Human TIMP 4-plex-this kit is only available in the premixed format

  • +Human XL Cytokine Discovery Panel

  • +Multi-species TGF-beta Panel(Human, Mouse, Rat and Porcine)

  • +Human Adhesion Molecule 4-plex-this kit is only available in the premixed format

  • +Human TIMP 4-plex - this kit is only available in the premixed format

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