Alkynyl-biotin

A biotin with a terminal alkyne group

Alkynyl-biotin is a form of biotin with a terminal alkyne group separated by two polyethylene glycol moieties. It is used to add biotin to other molecules bearing an azide group using a copper Cu(I)-catalyzed azide-alkyne cycloaddition reaction. Alkynyl-biotin has been used to add a biotin tag to a range of biochemicals, amino acids, proteins, and nucleotides that have been modified by the addition of an azide group.1,2,3 Alkynyl-biotin can also be used to capture sulfenic acid-modified proteins that have been tagged with the cell-permeable probe DAz-2 (Item No. 13382).4

468.6

≥95%

Biotin-PEG2-C4-Alkyne|Click Tag™ Alkynyl-biotin

O=C1N[C@]2([H])[C@H](CCCCC(NCCOCCOCCNC(CCCC#C)=O)=O)SC[C@]2([H])N1

C22H36N4O5S

1011268-28-2

≥ 4 years

DMF: 20 mg/ml DMSO: 20 mg/ml DMSO:PBS (pH 7.2) (1:1): 0.5 mg/ml

1.McClatchy, D.B.,Ma, Y.,Liu, C., et al. Pulsed azidohomoalanine labeling in mammals (PALM) detects changes in liver-specific LKB1 knockout mice. J. Proteome Res. 14(11), 4815-4822 (2015).
2.Wu, P.,Lu, M.-x.,Cui, X.-t., et al. A high-throughput-compatible assay to measure the degradation of endogenous Huntingtin proteins. Acta. Pharmacol. Sin. 37(10), 1307-1314 (2016).
3.Zayas, J.,Annoual, M.,Das, J.K., et al. Strain promoted click chemistry of 2- or 8-azidopurine and 5-azidopyrimidine nucleosides and 8-azidoadenosine triphosphate with cyclooctynes. Application to living cell fluorescent imaging. Bioconjug. Chem. 26(8), 1519-1532 (2015).
4.Leonard, S.E.,Reddie, K.G., and Carroll, K.S. Mining the thiol proteome for sulfenic acid modifications reveals new targets for oxidation in cells. ACS Chem. Biol. 4(9), 783-799 (2009).