TPEN

A transition metal chelator

As a cofactor of more than 300 enzymes, zinc is integral for a wide variety of cellular processes that affect most biological functions.1 Most notably, its bioavailability is important to the regulation of apoptosis, cell proliferation, and differentiation. TPEN is a cell permeant metal ion chelator that is used in zinc treatment studies to limit intra- and extracellular concentrations of the mineral.2 At a concentration as low as 6 µM, TPEN-induced zinc depletion decreases X-linked inhibitor of apoptosis (XIAP) levels in human PC-3 prostate cancer cells and promotes caspase-3 and -9 activation.3 At 25 µM, TPEN attenuates hypoxic pulmonary vasoconstriction in rodent isolated perfused lung.4

424.6

≥95%

4N(2-吡啶甲基)1,2-乙二胺≥95%杂质≤5%

TPEDA

c1ccc(nc1)CN(CCN(Cc1ccccn1)Cc1ccccn1)Cc1ccccn1

C26H28N6

16858-02-9

≥ 4 years

DMF: 1 mg/ml DMSO: 0.15 mg/ml Ethanol: 20 mg/ml Ethanol:PBS (pH 7.2) (1:10): 0.1 mg/ml

1.Beyersmann, D., and Haase, H. Functions of zinc in signaling, proliferation and differentiation of mammalian cells. BioMetals 14, 331-341 (2001).
2.Cho, Y.E.,Lomeda, R.A.R.,Ryu, S.H., et al. Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E₁ cells. Nutrition Research and Practice 1, 29-35 (2007).
3.Makhov, P.,Golovine, K.,Uzoo, R.G., et al. Zinc chelation induces rapid depletion of the X-linked inhibitor of apoptosis (XIAP) and sensitizes prostate cancer cells to TRAIL-mediated apoptosis. Cell Death and Differentiation 15(11), 1745-1751 (2008).
4.Bernal, P.J.,Leelavanichkul, K.,Bauer, E., et al. Nitric oxide mediated zinc release contributes to hypoxic regulation of pulmonary vascular tone. Circulation Research 102(12), 1575-1583 (2008).