α-Amanitin

无

A selective RNA polymerase II inhibitor

α-Amanitin is a cyclic peptide found in several species of the Amanita genus as well as other poisonous fungi.1 At 1, 10, and 50 µg/ml, respectively, α-amanitin inhibits RNA polymerase II, III, and IV, preventing translocation of the enzymes on DNA, which significantly slows the rate of transcription.2,3 RNA polymerase I is insensitive to its effects. This toxin is used in molecular biology to distinguish among types of RNA polymerase in a sample and to arrest RNA synthesis in transcription studies.2

919

≥90%

[L-天冬酰胺基-4-羟基-L-脯氨酰-(R-4，5-二羟基-L-异亮氨酸-6-羟基-2-巯基-L-色氨酰甘氨酰-L-异亮氨酸甘氨酰-L-半胱氨酸基]环状(4-8)-硫醚(R)-S-氧化物≥90%

O=C(N[C@@]1([H])[C@@H](C)[C@@H](O)CO)[C@@]2([H])N(C[C@H](O)C2)C([C@H](CC(N)=O)NC([C@@]([H])(NC(CNC([C@@]([C@H](CC)C)([H])NC(CN3)=O)=O)=O)CS(C4=C(C(C=CC(O)=C5)=C5N4)C[C@@H](C3=O)NC1=O)=O)=O)=O

C39H54N10O14S

23109-05-9

≥ 4 years

Water: 1mg/ml

1.Hallen, H.E.,Luo, H.,Scott-Craig, J.S., et al. Gene family encoding the major toxins of lethal Amanita mushrooms. Proceedings of the National Academy of Sciences of the United States of America 104(48), 19097-19101 (2007).
2.Gu, W.,Powell, W.,Mote, J., Jr., et al. Nascent RNA cleavage by arrested RNA polymerase II does not require upstream translocation of the elongation complex on DNA. J.Biol.Chem 268(34), 25604-25616 (1993).
3.Tipper, D.J. Inhibition of yeast ribonucleic acid polymerases by thiolutin. Journal of Bacteriology 116(1), 245-256 (1973).

2811