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IPTG (dioxane free)
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产品介绍
产品介绍
产品信息
宿主
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简单描述
An inducer of β-galactosidase activity
商品描述
IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon.1 It is used in the concentration range of 100 μM - 1.5 mM to induce protein expression where the gene is under the control of the lac operator and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.2
分子量
238.3
纯度
≥98%
组成成分
1-甲基乙基1-硫代-β-D-半乳吡喃糖苷≥98%杂质≤2%
背景
别名
Isopropyl β-thiogalactoside
结构式
O[C@@H]1[C@H](O)[C@@H](O)[C@H](SC(C)C)O[C@@H]1CO
分子式
C9H18O5S
CAS号
367-93-1
制备和贮存
保存
≥ 4 years
溶解方法
DMF: 30 mg/ml
DMSO: 20 mg/ml
Ethanol: 20 mg/ml
PBS (pH 7.2): 10 mg/ml
保存方式
-20°C
文献
文献
1.Kaster, K.R.,Burgett, S.G.,Rao, R.N., et al. Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing. Nucleic Acids Research 11(19), 6895-6911 (1983).
2.Sambrook, J. and Russell, D.W. Molecular cloning: A laboratory manual. 3, 1-21 (2001).
2.Sambrook, J. and Russell, D.W. Molecular cloning: A laboratory manual. 3, 1-21 (2001).
参考图片
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