IPTG (dioxane free)

An inducer of β-galactosidase activity

IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon.1 It is used in the concentration range of 100 μM - 1.5 mM to induce protein expression where the gene is under the control of the lac operator and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.2

238.3

≥98%

1-甲基乙基1-硫代-β-D-半乳吡喃糖苷≥98%杂质≤2%

Isopropyl β-​thiogalactoside

O[C@@H]1[C@H](O)[C@@H](O)[C@H](SC(C)C)O[C@@H]1CO

C9H18O5S

367-93-1

≥ 4 years

DMF: 30 mg/ml DMSO: 20 mg/ml Ethanol: 20 mg/ml PBS (pH 7.2): 10 mg/ml

1.Kaster, K.R.,Burgett, S.G.,Rao, R.N., et al. Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing. Nucleic Acids Research 11(19), 6895-6911 (1983).
2.Sambrook, J. and Russell, D.W. Molecular cloning: A laboratory manual. 3, 1-21 (2001).