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4nm Colloidal Gold-AffiniPure™ Goat Anti-Mouse IgG (H+L) (min X Hu,Bov,Hrs,Rb,Sw Sr Prot) (LM Grade)
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Target: Mouse
Host: Goat
Antibody Format: Whole IgG
Specificity: IgG (H+L)
Minimal Cross Reactivity: Human, Bovine, Horse, Rabbit, Swine Serum ProteinsConjugate: 4 nm Colloidal Gold
Product Category: ImmunoGold Complexes
Clonality: Polyclonal
RRID: AB_2338723
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
Histo-/Cyto-Chemistry:- 1:20-1:200
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
4 nm Colloidal Gold
LM Grade colloidal gold reagents are intended for light microscopy and immunoblotting. Since signal intensity is relatively independent of particle size when silver enhancement is used, we offer 4 nm particles because this size may penetrate tissues better than larger particles.
The 4 nm size may be used for electron microscopy in studies that require smaller particles since they are relatively uniform in size (coefficient of variation less than or equal to 15%), though small aggregates are not removed from this grade. Therefore, our 4 nm particles should only be used for electron microscopy with the
understanding that the presence of small aggregates may give misleading results.
A detailed protocol for silver enhancement is provided with all orders for LM Grade products. All reagents involved in the light-insensitive silver enhancement reaction can be prepared easily in the laboratory. However, those who wish to use commercially available silver enhancement kits can continue to do so. All LM Grade colloidal gold-protein complexes are freeze-dried in buffer with stabilizers and a preservative. After reconstitution, they may be frozen in aliquots for extended storage.