Filipin III

无

An active component of the filipin complex

Filipin III is a polyene and an active component of the polyene antibiotic filipin complex (Item No. 25073).1 It is active against various fungi, including B. dermatitidis, C. neoformans, H. capsulatum, C. albicans, and T. mentagrophytes (MICs = 1-10 μg/ml). Filipin III induces hemolysis of isolated rabbit erythrocytes (EC50 = 0.8 µg/ml).2 It induces apoptosis in HaCaT keratinocytes in a concentration-dependent manner.3 Filipin III binds to cholesterol and has been used to fluorescently label sterols in biological structures.4,5 The filipin complex has excitation maxima of 338 and 357 nm and an emission maxima of 480 nm.6

654.8

≥90%

4S，6S，8S，10R，12R，14R，16S，27S-八羟基-3R-(1R-羟基-己基)17，28r-二甲基-氧杂环十八烷-17E，19E，21E，21E，23E，25E-戊烯-2-酮≥90%杂质≤10%

O[C@@H]1/C(C)=C/C=C/C=C/C=C/C=C/[C@H](O)[C@@H](C)OC([C@@]([C@H](O)CCCCC)([H])[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C[C@@H](O)C1)=O

C35H58O11

480-49-9

≥ 2 years

DMF: 5 mg/ml DMSO: 10 mg/ml DMSO:PBS (pH 72) (1:4): 0.2 mg/ml Ethanol: 1 mg/ml

1.Castanho, M.A.R.B.,Coutinho, A., and Prieto, M.J.E. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. The Journal of Biological Chemisty 267, 204-209 (1992).
2.Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes. Cell Biology International Reports 8(7), 519-535 (1984).
3.Severs, N.J., and Robenek, H. Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. Biochimica et Biophysica Acta 737, 373-408 (1983).