Staurosporine

Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 μM, staurosporine induces cell apoptosis (2,3). 1.Ruegg, U.T. and Burgess, G.M. (1989) Trends Pharmacol. Sci. 10, 218-220. 2.Couldwell , W. T. et al. (1994) FEBS Lett. 345, 43-46. 3.Yue, T. L. et al. (1998) J. Mol. Cell. Cardiol. 30, 495-507.