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7-AAD Cell Staining Solution

7-AAD Cell Staining Solution

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品牌: CST
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产品介绍
产品介绍
产品信息
简单描述

7-AAD Cell Staining Solution is supplied in an aqueous buffered solution containing less than 0.09% sodium azide. It can be used to stain apoptotic cells.

商品描述

Product Usage Information

As a last step for flow cytometric assays, add 10 μl of 7-AAD Cell Staining Solution to 100 μl of sample. Incubate in the dark for 10 min at room temperature before analyzing cells on flow cytometer.

背景
背景
7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that binds poly(dG-dC) regions with an excitation maxima at 543 nm when bound to DNA (1). 7-AAD can be used as dead cell discriminating dye due to its exclusion from live cells with intact membranes (2). 1.Schmid, I. et al. (1992) Cytometry 13, 204-8. 2.Gill, J.E. et al. (1975) J Histochem Cytochem 23, 793-9.
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制备和贮存
保存方式

Store at 4ºC protected from light.

参考图片

Flow cytometric analysis of Jurkat cells, untreated (left) or treated with Etoposide #2200 (25 μM, overnight; right), using 7-AAD Cell Staining Solution.

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货号:
47501S
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