BD Pharmingen™ 7-AAD

7-Amino-Actinomycin D (7-AAD) is a convenient, ready-to-use nucleic acid dye that can be used in place of propidium iodide (PI) for the exclusion of nonviable cells in flow cytometric assays. The advantage of 7-AAD over PI is the ability to be used in conjunction with phycoerythrin (PE)- and fluorescein isothiocyanate (FITC)-labelled monoclonal antibodies in 2-color analysis, with minimal spectral overlap between 7-AAD, PE and FITC fluorescence emissions. The 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).  This reagent is used as a viability probe for methods of dead cell exclusion, based on light scatter and uptake of 7-AAD as detected in FL3.  This reagent does not require dilution.  Suggested quantity to use:  5 µl (0.25 µg)/test (1x10^6 cells) and incubate for 10 minutes before analysis.

(RUO)

Flow cytometry (Routinely Tested)

7-AAD

Aqueous buffered solution containing fetal bovine serum and ≤0.09% sodium azide.

Development References(1) 1. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV. Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry. 1992; 13(2):204-208. (Methodology: Flow cytometry).