Latrunculin A

无

An actin disruption agent

Actin disruption is used to study cell functions in vitro (e.g., migration, endocytosis) and in vivo (e.g., tumor cell invasion). Latrunculin A is a bioactive 2-thiazolidinone macrolide derived from sponges that sequesters G-actin and prevents F-actin assembly. It binds monomeric actin with 1:1 stoichiometry and can be used to block actin polymerization both in vitro (Kd = 0.2 μM) and in cells (0.5 μM, 30 min).{1,2,3 Latrunculin A (1-10 μM) causes depolymerization of tumor cell cytoskeleton within ten minutes.4 Overnight treatment of cells with latrunculin A (10 μM) strongly suppresses actin synthesis.5 Prolonged cell treatment blocks dexamethasone-induced changes in actin cytoskeleton with no effect on cell viability.6

421.6

≥95%

(4R)[(1R，4Z，8E，10Z，12S，15R，17R)-17-表示“羟基”5,12-二甲基-3-氧代-2,16-二氧双环[13 . 3 . 1]十九碳-4,8,10-特里恩-17-yl]-2-噻唑烷酮≥95%杂质≤5%

NSC 613011

C/C(CC/C=C/C=C\[C@@H](C)CC[C@@H]1C[C@@H]2C[C@@]([C@H]3NC(SC3)=O)(O)O1)=C/C(O2)=O

C22H31NO5S

76343-93-6

≥ 2 years

DMSO: 25 mg/ml Ethanol: 25 mg/ml

1.Coué, M.,Brenner, S.L.,Spector, I., et al. Inhibition of actin polymerization by latrunculin A. FEBS Letters 213(2), 316-318 (1987).
2.Yarmola, E.G.,Somasundaram, T.,Boring, T.A., et al. Actin-latrunculin A structure and function. The Journal of Biological Chemisty 275(36), 28120-28127 (2000).
3.Loubéry, S.,Wilhelm, C.,Hurbain, I., et al. Different microtubule motors move early and late endocytic compartments. Traffic 9, 492-509 (2008).
4.Hayot, C.,Debeir, O.,Van Ham, P., et al. Characterization of the activities of actin-affecting drugs on tumor cell migration. Toxicology and Applied Pharmacology 211, 30-40 (2006).
5.Lyubimova, A.,Bershadsky, A.D. and Ben-Ze'ev, A. Autoregulation of actin synthesis requires the 3'-UTR of actin mRNA and protects cells from actin overproduction. Journal of Cellular Biochemistry 76, 1-12 (1999).
6.Liu, X.,Wu, Z.,Sheibani, N., et al. Low dose latrunculin-A inhibits dexamethasone-induced changes in the actin cytoskeleton and alters extracellular matrix protein expression in cultured human trabecular meshwork cells. Experimental Eye Research 77, 181-188 (2003).

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