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Lipopolysaccharides (LPS)
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Lipopolysaccharides (LPS)

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CAS号:
93572-42-0
产品介绍
产品介绍
产品信息
产品详情
Immunology and Inflammation
简单描述

LPS is supplied as a lyophilized powder and is from E. coli serotype O111:B4. It is purified via phenol extraction.

SolubilitySoluble in PBS and H2O at 5mg/ml and cell culture medium at 1mg/ml.
商品描述

Product Usage Information

For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml sterile PBS. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 ng/ml for 15 min-24 hours. Soluble in PBS and cell culture medium at 5 mg/ml and 1 mg/ml, respectively.

组成成分
脂多糖 100%
背景
背景
Lipopolysaccharide (LPS), also known as endotoxin, is a major glycolipid constituent of the outer cell wall of gram-negative bacteria. LPS molecules typically consist of a strain-specific distal polysaccharide side chain known as the O-antigen, a hydrophilic core oligosaccharide, and a hydrophobic domain referred to as lipid A. Lipid A is covalently bound to the outer bacterial membrane and is responsible for the toxicity of LPS (1-3). LPS is a potent activator of the proinflammatory response in many mammalian cell types, including macrophages, monocytes, and endothelial cells. Investigators have demonstrated that LPS binds to the CD14/TLR4/MD2 receptor complex, which in turn induces inflammatory cytokines including TNF-α, Interleukin-1, and IFN-α, as well as numerous inflammatory proteins such as iNOS, NF-κB, RIG-1, and IRF-3 (4-6). 1.Raetz, C.R. and Whitfield, C. (2002) Annu Rev Biochem 71, 635-700. 2.Rietschel, E.T. et al. (1994) FASEB J 8, 217-25. 3.Maeshima, N. and Fernandez, R.C. (2013) Front Cell Infect Microbiol 3, 3. 4.Pålsson-McDermott, E.M. and O'Neill, L.A. (2004) Immunology 113, 153-62. 5.Wang, J. et al. (2008) J Immunol 180, 8011-9. 6.Zhang, F.X. et al. (1999) J Biol Chem 274, 7611-4.
翻译后修饰
unmodified
CAS号
93572-42-0
制备和贮存
保存方式

Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution store at -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

参考图片

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (3A6) Mouse mAb #12242 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM for 24 hr) and treated with 1 μg/ml LPS for the indicated times, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (C22B4) Rabbit mAb #4764 (lower).

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货号:
14011S
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询价
10mg
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