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ASCT2 (D7C12) Rabbit mAb

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ASCT2 (D7C12) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

ASCT2

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ASCT2 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Immunohistochemistry (Paraffin)	1:200 - 1:800
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800

同种型

Rabbit

分子量

49, 75

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Monkey,

目标/特异性

Specificity/Sensitivity

ASCT2 (D7C12) Rabbit mAb recognizes endogenous levels of total ASCT2 protein.

Species Reactivity:

Human, Monkey

敏感性

Endogenous

背景

Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6). 1.Utsunomiya-Tate, N. et al. (1996) J Biol Chem 271, 14883-90. 2.Bröer, S. (2008) Physiol Rev 88, 249-86. 3.Bode, B.P. et al. (2002) Am J Physiol Gastrointest Liver Physiol 283, G1062-73. 4.Fuchs, B.C. et al. (2007) Am J Physiol Cell Physiol 293, C55-63. 5.Nicklin, P. et al. (2009) Cell 136, 521-34. 6.Marin, M. et al. (2003) J Virol 77, 2936-45.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #69974.

数据库链接

Entrez-Gene ID

6510

UniProt ID

Q15758

参考图片

Flow cytometric analysis of fixed and permeabilized HT-29 cells using ASCT2 (D7C12) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

Western blot analysis of extracts from HT-29 cells, untreated or treated with peptide N-glycosidase F (PNGase F), and untreated SW620 cells using ASCT2 (D7C12) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded SW480 cell pellet (left, high-expressing) or SK-MEL-5 cell pellet (right, low-expressing) using ASCT2 (D7C12) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ASCT2 (D7C12) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using ASCT2 (D7C12) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using ASCT2 (D7C12) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using ASCT2 (D7C12) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using ASCT2 (D7C12) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ASCT2 (D7C12) Rabbit mAb.

Confocal immunofluorescent analysis of HT-29 cells using ASCT2 (D7C12) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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货号：

8057S

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20μl

100ul

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