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AffiniPure™ F(ab‘)2 Fragment Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot)

AffiniPure™ F(ab‘)2 Fragment Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot)

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品牌: Jackson
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反应种属:
Human
来源宿主:
山羊
产品介绍
产品介绍
产品信息
抗原名称
IgM, Fc5µ Fragment
宿主
山羊

Target: Human
Host: Goat
Antibody Format: F(ab')₂ Fragment
Specificity: IgM, Fc fragment specific
Minimal Cross Reactivity: Bovine Serum Proteins
Conjugate: Unconjugated
Product Category: F(ab')₂ Fragment Affinity-Purified Antibodies
Clonality: Polyclonal
RRID: AB_2337553

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with IgM from other species.

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

组成成分
山羊抗人IgM多克隆抗体,磷酸钠,氯化钠(抗体是免疫球蛋白,属于生物大分子,无生物安全风险,不具有对人传播传染病风险)
应用
反应种属
Human
交叉反应性
Bovine Serum Proteins
最小交叉反应
Bovine Serum Proteins
目标/特异性
IgM, Fc fragment specific
数据库链接
研究资源识别码
AB_2337553
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货号:
109-006-129
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询价
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1mg
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