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rhALDH1A1, CF (20 ug)
rhALDH1A1, CF (20 ug)

rhALDH1A1, CF (20 ug)

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品牌： R&D Systems
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    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    5869-DH

    FormulationSupplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
    ShippingThe product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 6 months from date of receipt, -70 °C as supplied.
    • 3 months, -70 °C under sterile conditions after opening.

    Recombinant Human Aldehyde Dehydrogenase 1-A1/ALDH1A1, CF Summary

    Product Specifications

    Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to produce NADH during the oxidation of propionaldehyde. The specific activity is >150 pmol/min/μg, as measured under the described conditions.
    Source
    E. coli-derived human Aldehyde Dehydrogenase 1-A1/ALDH1A1 protein
    Ser2-Ser501, with an N-terminal Met and 6-His tag
    Accession #
    P00352
    N-terminal Sequence
    Analysis
    Ser2
    Structure / Form
    Noncovalently-linked homotetramer
    Predicted Molecular Mass
    56 kDa
    SDS-PAGE
    57 kDa, reducing conditions
    背景
    别名
    acetaldehyde dehydrogenase 1,ALDC,aldehyde dehydrogenase 1 family, member A1,aldehyde dehydrogenase 1, soluble,Aldehyde Dehydrogenase 1A1,Aldehyde Dehydrogenase 1-A1,Aldehyde dehydrogenase family 1 member A1,Aldehyde dehydrogenase, cytosolic,aldehyde dehydrogenase, liver cytosolic,ALDH class 1,ALDH1,ALDH11,ALDH1A1,ALDH-E1,ALHDII,EC 1.2.1,EC 1.2.1.36,MGC2318,PUMB1,RALDH 1,RALDH1,retinal dehydrogenase 1,retinaldehyde dehydrogenase 1
    背景

    Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

    Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).

    References
    1. Marchitti, S.A. et al. (2008) Expert Opin. Drug Metab. Toxicol. 4:697.
    2. Zhao, D. et al. (1996) Eur. J. Biochem. 240:15.
    3. King, G. and Holmes, R. (1997) Adv. Exp. Med. Biol. 414:19.
    4. Jacobs, F.M. et al. (2007) Development 134:2673.
    5. Chute, J.P. et al. (2006) Proc. Natl. Acad. Sci. USA. 103:11707.
    6. Ueshima, Y. et al. (1993) Alcohol Alcohol. 1B:15.
    Entrez Gene IDs
    216 (Human)
    Alternate Names
    acetaldehyde dehydrogenase 1; ALDC; aldehyde dehydrogenase 1 family, member A1; aldehyde dehydrogenase 1, soluble; Aldehyde Dehydrogenase 1A1; Aldehyde Dehydrogenase 1-A1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH1; ALDH11; ALDH1A1; ALDH-E1; ALHDII; EC 1.2.1; EC 1.2.1.36; MGC2318; PUMB1; RALDH 1; RALDH1; retinal dehydrogenase 1; retinaldehyde dehydrogenase 1
    研究领域
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    货号：
    5869-DH-020
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    询价
    20ug
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