全部商品分类
Alexa Fluor® 488-conjugated Streptavidin
下载产品说明书 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询 Product: Streptavidin
Conjugate: Alexa Fluor® 488
Product Category: Streptavidin and Conjugates
RRID: AB_2337249
Conjugates of streptavidin are recommended for use with Biotin-SP-conjugated antibodies and Biotin-SP-conjugated ChromPure proteins.
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
Western Blot:- 0.5-2 µg / ml
Histo-/Cyto-Chemistry:- 0.5-2 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Alexa Fluor® 488
Amax: 493 Emax: 519nmAlexa Fluor® 488-conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media they are brighter than FITC, Cy2, and DyLight 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media.
