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alpha-E-Catenin Antibody
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alpha-E-Catenin Antibody

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品牌: CST
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分子量:
100
反应种属:
Human,Mouse,Rat,
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
alpha-E-Catenin
来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human α-E-Catenin. Antibodies are purified using protein A and peptide affinity chromatography.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunofluorescence (Immunocytochemistry)1:200
分子量
100
研究领域
癌症,发育生物学与干细胞研究,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,
目标/特异性

Specificity/Sensitivity

α-E-Catenin Antibody detects endogenous levels of alpha-E catenin protein. The antibody may cross-react with neuronal α-N-catenin. In some cell lysates, the antibody may cross-react with a 50 kDa band of unknown origin.

Species Reactivity:

Human, Mouse, Rat

敏感性
Endogenous
背景
背景
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). 1.Kobielak, A. and Fuchs, E. (2004) Nat Rev Mol Cell Biol 5, 614-25. 2.Yamada, S. et al. (2005) Cell 123, 889-901. 3.Drees, F. et al. (2005) Cell 123, 903-15. 4.Hwang, S.G. et al. (2005) J Biol Chem 280, 12758-65.
研究领域
癌症,发育生物学与干细胞研究,纤维化,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

数据库链接
Entrez-Gene ID
1495
UniProt ID
P35221

参考图片

Confocal immunofluorescent analysis of A-431 cells labeled with α-E-Catenin Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with phalloidin (red). Blue pseudocolor = TO-PRO-3 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines, using α-E-Catenin Antibody.

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货号:
3236T
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