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rhAMIGO3 Fc, CF (50 UG)
rhAMIGO3 Fc, CF (50 UG)

rhAMIGO3 Fc, CF (50 UG)

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品牌： R&D Systems
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    Scientific Data

    Bioactivity Recombinant Human AMIGO3 Fc Chimera Protein BioactivityView Larger

    Recombinant Human AMIGO3 Fc Chimera (Catalog # 9508‑AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-5 μg/mL.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    9508-AM

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 200 μg/mL in PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human AMIGO3 Fc Chimera Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-5 μg/mL.
    Source
    Chinese Hamster Ovary cell line, CHO-derived human AMIGO3 protein
    Human AMIGO3
    (Thr20-Pro378)
    Accession # Q86WK7    		 IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
    Accession #
    Q86WK7
    N-terminal Sequence
    Analysis
    Thr20
    Structure / Form
    Disulfide-linked homodimer
    Predicted Molecular Mass
    66 kDa
    SDS-PAGE
    83-103 kDa, reducing conditions
    背景
    别名
    adhesion molecule with Ig-like domain 3,ALI3,Alivin-3,AMIGO3,AMIGO-3,amphoterin-induced gene and ORF 3,amphoterin-induced protein 3,KIAA1851,MGC120552,Amphoterin-induced Gene and ORF 3
    背景

    Background: AMIGO3

    AMIGO3 (Amphoterin-induced protein 3), also called Alivin-3, is a 68 kDa single-pass type I membrane cell adhesion protein. AMIGO3 belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Human AMIGO3 cDNA encodes 504 amino acids (aa) including a 19 aa signal sequence, 264 aa extracelluar domain, 21 aa transmembrane domain and 100 aa cytoplasmic domain. Human AMIGO3 shares 77.2% and 79.1% aa sequence identity with mouse and rat AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 is expressed in brain tissues, also found in liver, kidney and spleen (3). AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (4). Our in-house data showed that AMIGO3 inhibited anti-CD3 induced IL-2 secretion on CD3+ cells, suggesting that AMIGO3 may be involved in T cell activation.

    References
    1. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
    2. SwissProt Accession # Q86WK7.
    3. Kuja-Panula J, et al. (2003) J Cell Biol 160:963.
    4. Ahmed Z, et al. (2013) PLoS One. 8:e61878.
    Long Name
    Amphoterin-induced Gene and ORF 3
    Entrez Gene IDs
    386724 (Human); 320844 (Mouse)
    Alternate Names
    adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 200 μg/mL in PBS.

    参考图片

    Recombinant Human AMIGO3 Fc Chimera (Catalog # 9508‑AM)inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody.The ED50 for this effect is 1-5 μg/mL.

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    货号：
    9508-AM-050
    一键复制
    询价
    50UG
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    当前规格1件起购 
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