Ammonium Bicarbonate, 1M in HPLC-Grade Water

Product Usage Information

1M Ammonium bicarbonate buffer in HPLC water is provided for use with Cell Signaling Technology's patented PTMScan® protocol. Secondary trypsin digestion of enriched LysC or Methylated peptides is recommended for all basophilic and methylation-specific motif antibodies. Use this 1M ammonium bicarbonate (20X) stock to prepare the final digestion buffer of 50mM ammonium bicarbonate with 5% acetonitrile. Consult the specific PTMScan® kit and protocol for more details on the appropriate protease before digesting any protein samples.