BD Pharmingen™ Annexin V Binding Buffer, 10X concentrate

The Annexin V Binding Buffer, 10X concentrate has been formulated for use in flow cytometric assays utilizing Annexin V.  The Annexin V Binding Buffer is a 10X concentrate composed of a 0.2 µm sterile filtered 0.1M Hepes (pH 7.4), 1.4M NaCl, and 25 mM CaCl2 solution.  Prior to staining cells, an appropriate quantity of a 1X working solution should be made by diluting the 10X concentrate 1:10 with distilled water.  Any remaining 1X solution at the end of the experiment should be discarded.

(RUO)

Flow cytometry (Routinely Tested)

Annexin V

Development References(1) 1. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995; 184(1):39-51. (Methodology: Apoptosis, Flow cytometry).