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Donkey Anti-Goat IgG (H&L) Antibody Alkaline Phosphatase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-705-125

品牌: Rockland
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
反应种属:
Goat
产品介绍
产品介绍
产品信息
荧光素标记
Alkaline Phosphatase (AP)

宿主
Donkey

免疫原
Goat IgG whole molecule

简单描述
Donkey Anti-Goat IgG (H&L) Antibody Alkaline Phosphatase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-705-125

商品描述
Donkey Anti-Goat IgG (H&L) Antibody Alkaline Phosphatase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-705-125

同种型
IgG

克隆类别
Polyclonal

纯度
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

浓度
1.0 mg/mL by UV absorbance at 280 nm

形式
Liquid (sterile filtered)

防腐剂
0.1% (w/v) Sodium Azide

缓冲体系
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

应用
实验应用
WB, ELISA;Anti-Goat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

反应种属
Goat

目标/特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

背景
别名
donkey anti-Goat IgG Antibody Alkaline Phosphatase Conjugation, donkey anti-Goat IgG Alk Phos Conjugated Antibody

背景
Anti-Goat IgG Alkaline Phosphatase Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

制备和贮存
存储溶液
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

保存方式
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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