全部商品分类
ANTI-HU HLA-E 3D12HLA-E AF488
下载产品说明书 下载COA 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询
种属反应
宿主/亚型
分类
类型
克隆号
偶联物
激发/发射光谱
形式
浓度
纯化类型
保存液
内含物
保存条件
运输条件
RRID
产品详细信息
Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.
Applications Reported: This 3D12HLA-E antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3D12HLA-E antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
靶标信息
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; lymphocyte antigen; MHC 1; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1
基因别名: EA1.2; EA2.1; HLA-6.2; HLA-E; HLAE; MHC; QA1
UniProt ID:(Human) P13747
Entrez Gene ID:(Human) 3133
参考图片
Normal human peripheral blood cells were stained with CD45RA Monoclonal Antibody, eFluor 450 (Product # 48-0458-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or HLA-E Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.
Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (blue histogram) or HLA-E Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Cells in the lymphocyte gate were used for analysis.