首页抗体一抗一抗

HU PCNA PC10 PUR MAB

1/6

HU PCNA PC10 PUR MAB

品牌： Thermo流式

用小程序，查商品更便捷

对比

咨询

产品介绍

产品使用步骤推荐

产品介绍

产品信息

克隆号

PC10 (3F81)

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

免疫组化 (IHC)

Assay-Dependent

查看1篇已发表文章1篇已发表文章

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

0.25 µg/test

查看1篇已发表文章1篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human,Mouse,Rat

已发表种属

Chordate

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

PC10 (3F81)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_468596

产品详细信息

Description: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36 kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase.

Applications Reported: The PC10 (a.k.a. 3F81) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: The PC10 (a.k.a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The PC10 antibody has also been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。