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Anti-Human Transferrin (RABBIT) Antibody - 600-401-034
品牌： Rockland
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
反应种属：
Human
产品介绍
产品介绍
产品信息
宿主
Rabbit
免疫原
Human Transferrin
简单描述
Anti-Human Transferrin (RABBIT) Antibody - 600-401-034
商品描述
Anti-Human Transferrin (RABBIT) Antibody - 600-401-034
同种型
IgG
克隆类别
Polyclonal
纯度
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
浓度
1.0 mg/mL by UV absorbance at 280 nm
形式
Liquid (sterile filtered)
防腐剂
0.01% (w/v) Sodium Azide
缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
基因
TF
应用
实验应用
ELISA;Anti-Human transferrin has been tested by ELISA and is assayed against 1.0 µg of Human Transferrin in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L] MX code #611-703-127 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:43,0
反应种属
Human
目标/特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigen resins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG.
背景
别名
rabbit anti-Transferrin antibody, Beta 1 metal binding globulin antibody, PRO1400 antibody, Serotransferrin antibody, Siderophilin antibody, TF antibody
背景
Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
保存方式
This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
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