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Anti-Human Transferrin (RABBIT) Antibody Peroxidase Conjugated - 600-403-034
品牌： Rockland
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This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.
反应种属：
Human
产品介绍
产品介绍
产品信息
荧光素标记
Peroxidase (HRP)
宿主
Rabbit
免疫原
Human Transferrin
简单描述
Anti-Human Transferrin (RABBIT) Antibody Peroxidase Conjugated - 600-403-034
商品描述
Anti-Human Transferrin (RABBIT) Antibody Peroxidase Conjugated - 600-403-034
同种型
IgG
克隆类别
Polyclonal
纯度
This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.
浓度
1.0 mg/mL by UV absorbance at 280 nm
形式
Lyophilized
防腐剂
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
基因
TF
应用
实验应用
ELISA;Anti-Human Transferrin Peroxidase conjugated antibody has been tested by ELISA and is designed for Immunoblotting, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
推荐用量
500 µL
反应种属
Human
目标/特异性
This reagent was purified from pooled rabbit sera obtained from rabbits immunized with Human Transferrin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified Human Transferrin and Human Serum.
样本类型以及上样量
500 µL
背景
别名
rabbit anti-Transferrin antibody peroxidase conjugation, HRP conjugated rabbit anti-Transferrin antibody, Beta 1 metal binding globulin antibody, PRO1400 antibody, Serotransferrin antibody, Siderophilin antibody, TF antibody
背景
Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.
制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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