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品牌: Rockland
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Mouse IgG and Mouse Serum.
反应种属:
Mouse
产品介绍
产品介绍
产品信息
荧光素标记
Rhodamine (TRITC)

宿主
Chicken

免疫原
Mouse IgG whole molecule

简单描述
Chicken Anti-Mouse IgG (H&L) Antibody Rhodamine Conjugated - 610-9002

商品描述
Chicken Anti-Mouse IgG (H&L) Antibody Rhodamine Conjugated - 610-9002

同种型
IgG

克隆类别
Polyclonal

纯度
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Mouse IgG and Mouse Serum.

浓度
1.0 mg/mL by UV absorbance at 280 nm

形式
Lyophilized

防腐剂
0.01% (w/v) Sodium Azide

缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

应用
实验应用
Dot Blot, FISH;Anti-Mouse IgG Rhodamine Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

推荐用量
1.0 mL

反应种属
Mouse

目标/特异性
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum, Mouse IgG and Mouse Serum.

样本类型以及上样量
1.0 mL

背景
别名
chicken anti-Mouse IgG rhodamine Conjugated Antibody, chicken anti-Mouse IgG Antibody TRITC Conjugation

背景
Anti-Mouse IgG Rhodamine Antibody generated in chicken detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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