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Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Fluorescein Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-102-042
Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Fluorescein Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-102-042
品牌： Rockland
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Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
反应种属：
Mouse
产品介绍
产品介绍
产品信息
荧光素标记
宿主
Goat
免疫原
Mouse IgG2b heavy chain
简单描述
Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Fluorescein Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-102-042
商品描述
Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Fluorescein Conjugated (Min X Bv, Hu, and Rb Serum Proteins) - 610-102-042
同种型
IgG
克隆类别
Polyclonal
纯度
Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
浓度
1.0 mg/mL by UV absorbance at 280 nm
形式
Lyophilized
防腐剂
0.01% (w/v) Sodium Azide
缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
应用
实验应用
Dot Blot;Anti-Mouse IgG2b Fluorescein antibody has been tested by dot blot and is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays.
推荐用量
1.0 mL
反应种属
Mouse
目标/特异性
Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
样本类型以及上样量
1.0 mL
背景
别名
goat anti-MOUSE IgG2b Antibody fluorescein Conjugation, goat anti-Mouse IgG2b (gamma 2b) FITC Conjugated Antibody
背景
Anti-Mouse IgG2b Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25% of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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