Rabbit anti-Phospho-PI3K p85 α(Y467/199) Polyclonal Antibody
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Rabbit anti-Phospho-PI3K p85 α(Y467/199) Polyclonal Antibody

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品牌: Absin
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    分子量:
    Detects a band of approximately 85kD
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    Detects a band of approximately 85kD
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    反应种属:
    Human, Mouse, Rat, Bovine
    Human, Mouse, Rat, Bovine
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    产品介绍
    产品信息
    荧光素标记
    Unlabeled
    纯化方式
    The antibody was purified by immunogen affinity chromatography.
    宿主
    Rabbit
    免疫原
    KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y467/199 of human PI3K p85 alpha/p55 gamma protein. The exact sequence is proprietary.
    商品描述

    Rabbit polyclonal antibody to PI3K p85 alpha/p55 gamma (Phospho-Y467/199).

    分子量
    Detects a band of approximately 85kD
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    克隆类别
    Polyclonal Antibody
    应用
    实验应用
    WB: 1:500-1:1000, IHC: 1:100-1:200, IF: 1:100-1:500, ICC: 1:100-1:500
    反应种属
    Human, Mouse, Rat, Bovine
    目标/特异性
    Recognizes endogenous levels of PI3K p85 alpha/p55 gamma protein only when phosphorylated at Y467/199.
    背景
    别名
    PI3K p85 alpha抗体, p55 gamma抗体
    翻译后修饰
    Phosphorylated
    制备和贮存
    存储溶液
    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    保存方式
    Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

    参考图片

    Western blot analysis of PI3K p85 alpha/p55 gamma (pY467/199) expression in HEK293T (A), NIH3T3 (B) whole cell lysates.

    Immunohistochemical analysis of PI3K p85 alpha/p55 gamma (pY467/199) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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    货号:
    abs147914-50ul
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