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品牌: Absin








分子量:
Detects a band of approximately 85kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Detects a band of approximately 85kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
反应种属:
Human, Mouse, Rat, Bovine
Human, Mouse, Rat, Bovine
产品介绍
产品信息
荧光素标记
Unlabeled

纯化方式
The antibody was purified by immunogen affinity chromatography.

宿主
Rabbit

免疫原
KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y467/199 of human PI3K p85 alpha/p55 gamma protein. The exact sequence is proprietary.

商品描述
Rabbit polyclonal antibody to PI3K p85 alpha/p55 gamma (Phospho-Y467/199).

分子量
Detects a band of approximately 85kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

克隆类别
Polyclonal Antibody

应用
实验应用
WB: 1:500-1:1000, IHC: 1:100-1:200, IF: 1:100-1:500, ICC: 1:100-1:500

反应种属
Human, Mouse, Rat, Bovine

目标/特异性
Recognizes endogenous levels of PI3K p85 alpha/p55 gamma protein only when phosphorylated at Y467/199.

背景
别名
PI3K p85 alpha抗体, p55 gamma抗体

翻译后修饰
Phosphorylated

制备和贮存
存储溶液
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

保存方式
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
参考图片
Western blot analysis of PI3K p85 alpha/p55 gamma (pY467/199) expression in HEK293T (A), NIH3T3 (B) whole cell lysates.
Immunohistochemical analysis of PI3K p85 alpha/p55 gamma (pY467/199) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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