ANTI-SOMATOSTATIN ICDCLS EF660

Immunohistochemistry of formalin-fixed paraffin embedded human pancreas tissue using 5 µg/mL Anti-Somatostatin eFluor® 660 (white), 10 µg/mL Anti-Glucagon Alexa Fluor® 488 (green), and 0.5 µg/mL Anti-Insulin eFluor® 570 (red). Nuclei are stained with DAPI (blue).

Figure 2 DBP Alters alpha Cell and delta Cell Number and Size (A and B) Cell resolution reconstruction of pancreatic sections reveals no differences in alpha cell and beta cell mass in DBP +/+ and DBP -/- mice (A), quantified in bar graph (B) (scale bar represents 530 mum; representative images are shown; inset is a zoom showing maintenance of cellular resolution in a single image; n = 9-12 sections from 3 to 4 animals; unpaired t test). (C-I) Morphological analyses of DBP -/- islets (C) reveal increased alpha cell number (D) and decreased alpha cell size (E) (representative images shown in right panel), but normal area occupied (F). By contrast, beta cell number is increased (G), although beta cell size (H) (representative images shown in right panel) and area (I) are unchanged (scale bar in D represents 85 mum; scale bars in E and H represent 10 mum; n = 24-45 islets from 3 to 4 animals; E and H are zooms of C to better show alpha cell and beta cell size; DAPI is shown in blue; unpaired t test). (J-M) delta cell proportion (J and K; n = 55-79 islets from 4 to 5 animals) and size (L and M; n = 29 to 30 islets from 3 animals) are decreased in DBP -/- islets (scale bar in J represents 85 mum; scale bar in L represents 10 mum; representative images are shown; L is a zoom of J to better show delta cell size; unpaired t test). (N) Expression levels of the alpha cell differentiation markers Arx , Pax6 , Pou3f4 , and Irx2 are similar in DBP +/- and DBP -/- islets (n = 3-10 animals;