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Anti-Streptavidin (RABBIT) Antibody Biotin Conjugated (BULK ORDER) - 200-4695
品牌： Rockland
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Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
反应种属：
Streptavidin
产品介绍
产品介绍
产品信息
荧光素标记
Biotin
宿主
Rabbit
免疫原
Streptavidin (Streptomyces avidinii)
简单描述
Anti-Streptavidin (RABBIT) Antibody Biotin Conjugated (BULK ORDER) - 200-4695
商品描述
Anti-Streptavidin (RABBIT) Antibody Biotin Conjugated (BULK ORDER) - 200-4695
同种型
IgG
克隆类别
Polyclonal
纯度
Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
形式
Lyophilized
防腐剂
0.01% (w/v) Sodium Azide
缓冲体系
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
应用
实验应用
IF, Dot Blot, FISH, Multiplex;Biotin Conjugated Anti-Streptavidin Antibody has been tested by dot blot and is suitable to be assayed by ELISA for the detection of streptavidin in a standard ELISA using Peroxidase as a reporter. A working dilution of 1:10,000 to 1:400,000 of the reconstitution concentration is suggested for this
推荐用量
1.0 mL
反应种属
Streptavidin
目标/特异性
Streptavidin Antibody Biotin Conjugated was prepared from monospecific antiserum by delipidation, defibrination, salt fractionation and ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Streptavidin. No reaction was observed against Avidin.
样本类型以及上样量
1.0 mL
背景
别名
rabbit anti-Streptavidin Antibody biotin Conjugation, biotin conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin BAC Antibody
背景
Anti-Streptavidin Antibody is Biotin Conjugated and detects streptavidin. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Streptavidin and avidin bind biotin with high affinity (Kd of 10−14 mol/l to 10−15 mol/l) and thus biotinylated proteins of interest can be enriched from a sample due to this highly stable interaction. Biotin conjugated anti-streptavidins are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications. This antibody reagent can bind to streptavidin through the antibody F(ab) or can be bound by streptavidin through the high affinity biotin-streptavidin interaction.
制备和贮存
存储溶液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
保存方式
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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