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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #89872.
参考图片
Flow cytometric analysis of human whole blood using Arginase-1 (D4E3M™) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Analysis was performed on cells in the granulocyte gate.
Western blot analysis of extracts from mouse and rat liver using Arginase-1 (D4E3M™) XP® Rabbit mAb.
Western blot analysis of extracts from mouse liver and mouse small intestine using Arginase-1 (D4E3M™) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Simple Western™ analysis of extracts (0.1 mg/mL) from mouse liver tissue using Arginase-1 (D4E3M™) XP® Rabbit mAb #93668. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb performed on the Leica® BOND™ Rx.
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded normal human liver using Arginase-1 (D4E3M™) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Arginase-1 (D4E3M™) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse liver using Arginase-1 (D4E3M™) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse liver (positive; left) or small intestine (negative; right) using Arginase-1 (D4E3M™) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) using Arginase-1 (D4E3M™) XP® Rabbit mAb (green). BMDMs were differentiated with M-CSF (20 ng/ml, 7 days) and activated with either IL-4/cAMP (20 ng/ml, 0.5 mM, 24 hours; left) or LPS/IFNγ (50 ng/ml, 20 ng/ml, 24 hours; right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.