一、报警压设置
分子筛在连接仪器之前需要设置报警压。如果超压会对柱子造成不可逆损伤。
● 有柱位阀的仪器：根据说明书或者以下表格设置柱前压和delta压；
● 没有柱位阀的仪器：系统压设置为delta column pressure+空载压力和pre column pressure两者中较小的那个数值。
注意：如果仪器在低温运行或者样品比较粘稠，适当降低报警压数值。
| 柱子名称 | Pre column pressure | delta column pressure |
| Superdex 30/75/200 increase 10/300 | 5 MPa |
3 MPa |
| Superose 6 Increase 10/300 | ||
| Hiload 16/600 Superdex 30/75/200 pg | 0.5 MPa |
0.3 MPa |
| Hiload 26/600 Superdex 30/75/200 pg | ||
| Superdex 30/75/200 increase 3.2/300 | 5 MPa |
2 MPa |
| Superose 6 Increase 3.2/300 | ||
| Superdex 30/75/200 increase 5/150 | 10 MPa |
3 MPa |
| Superose 6 Increase 5/150 |
设置报警压软件操作：
Manual——Manual Instructions——Alarms——Alarm pre column pressure、Alarm delta column pressure
二、可耐受溶液范围
Superdex Increase，Superose Increase，Hiload Superdex系列
Long term use
Long term use 指填料在这些溶液中可以保持长时间稳定，并对填料没有不良的副作用。
• All commonly used aqueous buffers, pH 3 to 12
• Trifluoroacetic acid, up to 0.2%
• Isopropanol, up to 5%
• Dithiothreitol (DTT), up to 5 mM
• Acetonitrile, up to 10%
• Formic acid, up to 1%
• Urea, up to 8 M
• Guanidine hydrochloride, up to 6 M
• Ionic and non-ionic detergents, e.g., 1% SDS
• Methanol, up to 10%
Short term use
Short term use 指可用于层析柱清洗，再生和消毒。
• Sodium hydroxide, up to 1 M
• Ethanol, up to 70%
• Isopropanol, up to 30%
• Hydrochloric acid, up to 0.1 M
• Trifluoroacetic acid, up to 1%
• Dimethyl sulfoxide (DMSO), up to 10%
• Acetonitrile, up to 30%
• Methanol, up to 70%
• Formic acid, up to 70%
• Acetic acid, up to 1M
Avoid:
• Oxidizing agents
• Non-filtered samples and eluents
更多纯化相关资料和讲座，请进入优宁维蛋白纯化子网站查看：https://www.univ-bio.com/protein-home.html
快速筛选出想要的纯化产品，点击进入纯化柱/填料筛选器：https://www.univ-bio.com/tooles/proteinTool2.html
