首页 抗体 一抗 一抗
ASC/TMS1 (D2W8U) Rabbit mAb
ASC/TMS1 (D2W8U) Rabbit mAb

ASC/TMS1 (D2W8U) Rabbit mAb

分享
品牌： CST
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    分子量：
    22
    22
    反应种属：
    Mouse
    Mouse
    展开
    产品介绍
    产品信息
    抗原名称
    ASCTMS1
    来源纯化
    使用重组小鼠 ASC/TMS1 蛋白对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying ASC mouse. Cited in 240 publications. Validated for WB, IP, IHC, IF, IF, F. Available in 2 sizes. Highly specific and rigorously validated in-house, ASC/TMS1 (D2W8U) Rabbit Monoclonal Antibody (CST #67824) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:100
    Immunohistochemistry (Paraffin)1:100 - 1:400
    Immunofluorescence (Frozen)1:400 - 1:1600
    Immunofluorescence (Immunocytochemistry)1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized)1:400 - 1:1600
    分子量
    22
    研究领域
    纤维化,免疫学和肿瘤学,神经科学
    应用
    反应种属
    Mouse
    目标/特异性

    Specificity/Sensitivity

    ASC/TMS1 (D2W8U) Rabbit mAb 可识别 ASC/TMS1 总蛋白的内源水平。

    物种反应性：

    小鼠

    敏感性
    Endogenous
    背景
    背景
    TMS1（甲基化诱导沉默的靶标）/ASC（含有 CARD 且与凋亡相关的斑点样蛋白）（也被称为 PYCARD 和 CARD5）是一个包含一个 N 末端 热蛋白域 (PYD) 和一个 C 末端 半光天冬梅酶募集区域 (CARD) 的 22-kDa 促凋亡蛋白 (1-2)。ASC/TMS1 基因最早被发现在乳腺癌细胞 (2) 中出现异常甲基化和沉默，随后被发现在其他一些癌细胞中沉默，包括卵巢癌 (3)、胶质母细胞瘤 (4)、黑色素瘤 (5)、胃癌 (6)、肺癌 (7) 和前列腺癌 (8)。促凋亡/炎症刺激会诱导 ASC/TMS1 表达 (9)。凋亡期间，ASC/TMS1 从胞质重分布到线粒体，并且结合线粒体 Bax，从而引发细胞色素 c 释放和随后的凋亡 (10)。ASC/TMS1 还被发现是炎症信号转导的一个关键组分，在炎症信号转导中受到促炎性信号刺激时结合并激活 caspase-1 (11)。 1.Masumoto, J. et al. (1999) J Biol Chem 274, 33835-8. 2.Conway, K.E. et al. (2000) Cancer Res 60, 6236-42. 3.Terasawa, K. et al. (2004) Clin Cancer Res 10, 2000-6. 4.Stone, A.R. et al. (2004) Am J Pathol 165, 1151-61. 5.Guan, X. et al. (2003) Int J Cancer 107, 202-8. 6.Moriai, R. et al. (2002) Anticancer Res 22, 4163-8. 7.Virmani, A. et al. (2003) Int J Cancer 106, 198-204. 8.Das, P.M. et al. (2006) Mol Cancer 5, 28. 9.Strong, R. et al. (1991) Brain Res 542, 23-8. 10.Ohtsuka, T. et al. (2004) Nat Cell Biol 6, 121-8. 11.Srinivasula, S.M. et al. (2002) J Biol Chem 277, 21119-22.
    研究领域
    纤维化,免疫学和肿瘤学,神经科学
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #37953.
    数据库链接
    Entrez-Gene ID
    66824
    UniProt ID
    Q9EPB4

    参考图片

    Western blot analysis of extracts from J774A.1 and Raw 264.7 cells using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Immunoprecipitation of ASC/TMS1 from J774A.1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ASC (D2W8U) Rabbit mAb (Mouse Specific). Western blot analysis was performed using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific).

    Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) either untreated (upper left) or treated with LPS (50 ng/ml, 4 hr, middle) or LPS followed by ATP (5 mM, 45 min, upper right), and J774A.1 (lower left) or Raw 264.7 (lower right) cells, using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and ATP (white arrows).

    Flow cytometric analysis of Raw264.7 cells (blue) and J774A.1 cells (green) using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    67824T
    一键复制
    询价
    优宁维现货
    20ul
    100ul
    抗体送U盘
    详情
    选择数量
    当前规格1件起购 
    配送至
    预计3-4个工作日送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价