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ASIC1 ANTIBODY
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Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. ASIC1 is permeable for Ca(2+), Li(+) and K(+) and is expressed in dorsal root ganglia, sciatic nerves, olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. It is diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ACCN2 variant 3; acid sensing (proton gated) ion channel 1; acid sensing ion channel; acid sensing ion channel 1; acid-sensing (proton-gated) ion channel 1; Acid-sensing ion channel; Acid-sensing ion channel 1; acid-sensing ion channel 1a protein; Amiloride-sensitive cation channel 2, neuronal; ASIC1; BNaC2; Brain sodium channel 2; Cation channel, amiloride-sensitive, neuronal, 2; degenerin 2; hBNaC2; proton gated cation channel ASIC1
基因别名: ACCN2; AI843610; ASIC; ASIC1; ASIC1a; B530003N02Rik; BNAC2
UniProt ID:(Human) P78348, (Mouse) Q6NXK8, (Rat) P55926
Entrez Gene ID:(Human) 41, (Mouse) 11419, (Rat) 79123
参考图片
Expression of ASIC1 in rat C6 glioma cells - Immunocytochemical staining of fixed and permeabilized C6 glioma cells. A. Cells were stained using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:200) followed by Anti-guinea pig-AlexaFluor-594 secondary Antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
Multiplex staining of ASIC1 and parvalbumin in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:300). A. ASIC1 (green) is detected in the hilus of the dentate gyrus (arrows). B. The same section was stained with Anti-parvalbumin (red). C. Merge of the two images shows partial colocalization (orange, see arrows). Cell nuclei in the same section were stained with DAPI (Blue).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600). 2. Guinea pig Anti-ASIC1 Antibody , preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Guinea pig Anti-ASIC1 Antibody (#ASC-014-GP), (1:600).3,4. Guinea pig Anti-ASIC1 Antibody , preincubated with ASIC1 Blocking Peptide (#BLP-SC014).