CST_ATF-4(D4B8) Rabbit mAb_优宁维(univ)在线
现货 ATF-4(D4B8) Rabbit mAb
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货号: 11815S 品牌: CST 库存: 公司现货
规格: 100ul
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价格: ¥4043.00

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产品信息
来源纯化
使用与人 ATF-4 蛋白羧基末端周围的残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。
宿主
Rabbit
应用
敏感性
内源性
背景
背景
ATF-4 是激活转录因子/ cAMP 反应元件结合蛋白家族的一员,在 PERK 和 eIF2α 内质网应激反应通路中发挥作用 (1-3)。内质网应激会抑制多数 mRNA 的翻译,但也选择性地刺激某些 mRNA(如 ATF-4)的翻译 (2)。ATF-4 的诱导表达会增加基因表达,这对于从内质网应激状态中恢复正常是非常重要的 (4)。 Fawcett, T.W. et al. (1999) Biochem J 339 (Pt 1), 135-41. Harding, H.P. et al. (2000) Mol Cell 6, 1099-108. van Huizen, R. et al. (2003) J Biol Chem 278, 15558-64. Yusta, B. et al. (2006) Cell Metab 4, 391-406.
分子量
49
Entrez-Gene ID
468
UniProt ID
P18848
抗原
ATF-4

参考图片

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or tunicamycin-treated (2 μg/ml, 8 hr; right), using ATF-4 (D4B8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

共聚焦免疫荧光分析HeLa 细胞,未处理(左图)或衣霉素处理 (2 μg/ml, 8 hr; 右图),所用抗体为 ATF-4 (D4B8) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。

Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or tunicamycin-treated (2 μg/ml, 8 hr; +), using ATF-4 (D4B8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot方法检测293和HeLa细胞提取物:未经处理 (-) 和衣霉素处理(2 μg/ml, 8 hr; +),使用的抗体是ATF-4 (D4B8) Rabbit mAb兔单抗 (上图) 或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图)。

Immunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ATF-4 (D4B8) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ATF-4 (D4B8) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

免疫沉淀方法检测293细胞提取物ATF-4蛋白,使用衣霉素处理(2 μg/ml, 8 hr),使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或ATF-4 (D4B8) Rabbit mAb (泳道3)。泳道1为10%对照。使用ATF-4 (D4B8) Rabbit mAb 进行Western blot方法分析,二抗是Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 和Anti-mouse IgG, HRP-linked Antibody #7076。

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and 5 µl of ATF-4 (D4B8) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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