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ATF-4 (D4B8) Rabbit mAb
ATF-4 (D4B8) Rabbit mAb

ATF-4 (D4B8) Rabbit mAb

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品牌： CST
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    分子量：
    49
    49
    反应种属：
    Human,Mouse,Rat,
    Human,Mouse,Rat,
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    产品介绍
    产品信息
    抗原名称
    ATF-4
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATF-4 protein.
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying ATF-4. Cited in 769 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R, C&T. Highly specific and rigorously validated in-house, ATF-4 (D4B8) Rabbit Monoclonal Antibody (CST #11815) is ready to ship.
    商品描述

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using the CUT&RUN Assay Kit #86652. The CUT&Tag dilution was determined using 100,000 cells in a total reaction volume of 100 μl.

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:50
    Immunofluorescence (Immunocytochemistry)1:100 - 1:400
    Chromatin IP1:200
    Chromatin IP-seq1:200
    CUT&RUN1:200
    CUT&Tag1:200
    分子量
    49
    研究领域
    癌症,纤维化,代谢,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,
    目标/特异性

    Specificity/Sensitivity

    ATF-4 (D4B8) Rabbit mAb recognizes endogenous levels of total ATF-4 protein.

    Species Reactivity:

    Human, Mouse, Rat

    敏感性
    Endogenous
    背景
    背景
    ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4). 1.Fawcett, T.W. et al. (1999) Biochem J 339 (Pt 1), 135-41. 2.Harding, H.P. et al. (2000) Mol Cell 6, 1099-108. 3.van Huizen, R. et al. (2003) J Biol Chem 278, 15558-64. 4.Yusta, B. et al. (2006) Cell Metab 4, 391-406.
    研究领域
    癌症,纤维化,代谢,神经科学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
    数据库链接
    Entrez-Gene ID
    468
    UniProt ID
    P18848

    参考图片

    Confocal immunofluorescent analysis of HeLa cells, untreated (left) or tunicamycin-treated (2 μg/ml, 8 hr; right), using ATF-4 (D4B8) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

    共聚焦免疫荧光分析HeLa 细胞，未处理(左图)或衣霉素处理 (2 μg/ml, 8 hr; 右图)，所用抗体为 ATF-4 (D4B8) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。

    Western blot analysis of extracts from 293 and HeLa cells, untreated (-) or tunicamycin-treated (2 μg/ml, 8 hr; +), using ATF-4 (D4B8) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Western blot方法检测293和HeLa细胞提取物：未经处理 (-) 和衣霉素处理(2 μg/ml, 8 hr; +)，使用的抗体是ATF-4 (D4B8) Rabbit mAb兔单抗 (上图) 或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图)。

    Immunoprecipitation of ATF-4 from extracts of 293 cells, treated with tunicamycin (2 μg/ml, 8 hr), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ATF-4 (D4B8) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ATF-4 (D4B8) Rabbit mAb. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

    免疫沉淀方法检测293细胞提取物ATF-4蛋白，使用衣霉素处理(2 μg/ml, 8 hr)，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或ATF-4 (D4B8) Rabbit mAb (泳道3)。泳道1为10%对照。使用ATF-4 (D4B8) Rabbit mAb 进行Western blot方法分析，二抗是Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 和Anti-mouse IgG, HRP-linked Antibody #7076。

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and 5 µl of ATF-4 (D4B8) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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    货号：
    11815S
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    20μl
    100ul
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