Atg12 (D88H11) Rabbit mAb

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). 1.Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2.Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3.Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4.Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5.Mizushima, N. et al. (1998) Nature 395, 395-8. 6.Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7.Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8.Shintani, T. et al. (1999) EMBO J 18, 5234-41.