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Atg5 (D5F5U) Rabbit mAb

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Atg5 (D5F5U) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

Atg5

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu265 of human Atg5 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:50 - 1:250
Immunoprecipitation	1:100

同种型

Rabbit IgG

分子量

内毒素水平

猴, 非洲爪蟾蜍, 犬 , 猪

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

应用

反应种属

Human,Mouse,Rat,

预测反应种属

Monkey,Xenopus,Dog,Pig

目标/特异性

Specificity/Sensitivity

Atg5 (D5F5U) Rabbit mAb recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). 1.Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21. 2.Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18. 3.Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88. 4.Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92. 5.Mizushima, N. et al. (1998) Nature 395, 395-8. 6.Suzuki, K. et al. (2001) EMBO J 20, 5971-81. 7.Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79. 8.Shintani, T. et al. (1999) EMBO J 18, 5234-41.

研究领域

癌症,细胞生物学,纤维化,代谢,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

9474

UniProt ID

Q9H1Y0

参考图片

Immunoprecipitation of Atg5 from PANC-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Atg5 (D5F5U) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Atg5 (D5F5U) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross-reactivity with rabbit IgG.

Western blot analysis of extracts from various cell lines using Atg5 (D5F5U) Rabbit mAb.

Western blot analysis of extracts from wild-type MEFs (wt) or MEFs from Atg5 knockouts (Atg5-/-) using Atg5 (D5F5U) Rabbit mAb (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Atg5-/- MEFs were kindly provided by Dr. Ramnik Xavier, Massachusetts General Hospital, Harvard Medical School, Boston, MA.

Simple WesternTM analysis of lysates (0.1 mg/mL) from HeLa cells using Atg5 (D5F5U) Rabbit mAb #12994. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

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货号：

12994S

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