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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Aurora A
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro70 of human Aurora A protein.
宿主
Rabbit
简单描述
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Aurora A (D3E4Q) Rabbit mAb #14475.
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
同种型
Rabbit IgG
克隆号
D3E4Q
研究领域
代谢,神经科学,
应用
反应种属
Human
目标/特异性
Specificity/Sensitivity
Aurora A (D3E4Q) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Aurora A protein.
Species Reactivity:
Human
敏感性
Endogenous
背景
背景
Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).
1.Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
2.Zhou, H. et al. (1998) Nat Genet 20, 189-93.
3.Sen, S. et al. (1997) Oncogene 14, 2195-200.
4.Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
5.Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
6.Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
研究领域
代谢,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
数据库链接
Entrez-Gene ID
6790
UniProt ID
O14965
参考图片
Flow cytometric analysis of Jurkat cells using Aurora A (D3E4Q) Rabbit mAb (PE Conjugate) and DRAQ5® #4084 to measure DNA content.
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