B cell Antibody (clone Ki-B1R) LS-C96406

Affinity purified

B cell

Mouse

Lymphocytes

B cell antibody LS-C96406 is an unconjugated mouse monoclonal antibody to rat B cell. Validated for IHC.

Monoclonal

IHC-P, IHC-Fr

IHC - Paraffin (1:10) IHC - Frozen (1:400)

Rat

Ki-B1R recognizes a surface antigen of 47kDa molecular weight, which appears as a single band under reducing conditions in SDS-PAGE. The epitope has not been further characterized. Antigen distribution: Isolated cells: In a test comparing the immunophenotype of rat blood lymphocytes, Ki-B1R recognised 19% + 3% of peripheral blood lymphocytes (n=5). In the same test, 18% +/- 3% of the lymphocytes stained positive for sIg, whereas 79% +/- 3% stained positive with Ki-T1R, the rat pan T-cell marker. Tissue sections: Ki-B1R shows a staining pattern similar to that of anti-rat immunoglobulin markers. Follicular mantle B- lymphocytes are strongly positive whereas staining of germinal centre B-lymphocytes depends on the immunological status of the lyphoid organ. In the gastrointestinal tract, the typical mucosa-associated lymphoid tissue (MALT) selectively displays the B-follicles, mostly those containing prominent germinal centres. The reactivity pattern of Ki-B1R does not change when applied in vivo. Specificity: Rat: mature B-lymphocytes, plasma cells. Other species: not tested.