BD IMag™ Human B Lymphocyte Enrichment Set - DM

Enrichment of B lymphocytes from PBMC derived from two different donors. Leukocytes were labeled with the BD IMag™ Human B Lymphocyte Enrichment Set - DM (Cat. No. 558007) and separated using the BD IMag™ Cell Separation Magnet (Cat. No. 552311). Cells were stained with APC Mouse Anti-Human CD19 (Cat. no. 555415) to detect B lymphocytes and a mixture of FITC Mouse Anti- Human CD3 (Cat. No. 555332) and CD43 (Cat. No. 555475) to detect non-B leukocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution (Cat. No.556463), Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. The percentages of CD19+ CD3-CD43- B lymphocytes in each sample is given. The data from two separate donors (top vs. bottom panels) demonstrate that the additional separation step increases the purity of the enriched cells when the donor has a low frequency of B lymphocytes.

Enrichment of B lymphocytes from PBMC derived from two different donors. Leukocytes were labeled with the BD IMag™ Human B Lymphocyte Enrichment Set - DM (Cat. No. 558007) and separated using the BD IMag™ Cell Separation Magnet (Cat. No. 552311). Cells were stained with APC Mouse Anti-Human CD19 (Cat. no. 555415) to detect B lymphocytes and a mixture of FITC Mouse Anti- Human CD3 (Cat. No. 555332) and CD43 (Cat. No. 555475) to detect non-B leukocytes. Dead cells were excluded by staining with Propidium Iodide Staining Solution (Cat. No.556463), Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. The percentages of CD19+ CD3-CD43- B lymphocytes in each sample is given. The data from two separate donors (top vs. bottom panels) demonstrate that the additional separation step increases the purity of the enriched cells when the donor has a low frequency of B lymphocytes.