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产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
Bak
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly75 of human Bak protein.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
同种型
Rabbit IgG
分子量
25
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性
Specificity/Sensitivity
Bak (D4E4) Rabbit mAb recognizes endogenous levels of total Bak protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Bak is a proapoptotic member of the Bcl-2 family (1). This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3). Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
1.Gross, A. et al. (1999) Genes Dev. 13, 1899-1911.
2.Wei, M.C. et al. (2001) Science 292, 727-730.
3.Zong, W.X. et al. (2001) Genes Dev. 15, 1481-1486.
4.Degenhardt, K. et al. (2002) J. Biol. Chem. 277, 14127-14134.
5.Wei, M.C. et al. (2000) Genes Dev. 14, 2060-2071.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #87579.
数据库链接
Entrez-Gene ID
578
UniProt ID
Q16611
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