Puma Antibody

Puma (p53 upregulated modulator of apoptosis) is a "BH3-only" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The "BH3-only" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the puma gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cytochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the puma gene impairs p53-mediated apoptosis and tumor suppression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4). 1.Yu, J. et al. (2001) Mol Cell 7, 673-82. 2.Nakano, K. and Vousden, K.H. (2001) Mol Cell 7, 683-94. 3.Bouillet, P. and Strasser, A. (2002) J Cell Sci 115, 1567-74. 4.Jeffers, J.R. et al. (2003) Cancer Cell 4, 321-8. 5.Hemann, M.T. et al. (2004) Proc Natl Acad Sci U S A 101, 9333-8. 6.Yu, J. et al. (2003) Proc Natl Acad Sci U S A 100, 1931-6. 7.Villunger, A. et al. (2003) Science 302, 1036-8.