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NucA MAb (Cl 1028905 (25 ug)
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NucA MAb (Cl 1028905 (25 ug)

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品牌: R&D Systems
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来源宿主:
Mouse IgG2b
产品介绍
产品介绍
产品信息
荧光素标记
宿主
Mouse IgG2b
克隆号
1028905

Scientific Data

Western Blot NucA nucleaseView Larger

Detection of NucA nuclease by Western Blot. Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western Detection of NucA nuclease by Simple WesternView Larger

Detection of NucA nuclease by Simple WesternTM. Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100633). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

NucA nuclease Antibody Summary

Specificity
Detects NucA nuclease in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 1028905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived s. marcescens NucA nuclease
Met1-Asn266
Accession # P13717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Recombinant NucA nuclease Protein
Simple Western
10 µg/mL
Recombinant NucA nuclease Protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

背景
别名
DcD,NucA nuclease,NucA nuclease
背景

Background: NucA nuclease

Long Name
NucA nuclease
Entrez Gene IDs
23387471 (Bacterial)
Alternate Names
DcD; NucA nuclease
研究领域
制备和贮存
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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货号:
MAB100633-SP
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