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PathScan RP beta-Amyloid (1-42) Sandwich ELISA Kit
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产品介绍
产品介绍
产品信息
简单描述
The rapid protocol (RP) PathScan® RP β-Amyloid (1-42) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of the Aβ-42 isoform of β-amyloid in a reduced assay time of 1.5 hours. Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with Aβ-42 in a single step. The plate is then extensively washed and TMB reagent is added for signal development. The magnitude of absorbance for the developed color is proportional to the quantity of Aβ-42. Learn more about all of your ELISA kit options here.
*Antibodies in this kit are custom formulations specific to kit.
组合货号
9803S&27029CA
研究领域
神经科学
应用
反应种属
Human
目标/特异性
Specificity/Sensitivity
The PathScan RP β-Amyloid (1-42) Sandwich ELISA Kit detects endogenous levels of the Aβ-42 isoform of β-amyloid peptides, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human
背景
背景
Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).
1.Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
2.Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.
3.Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.
4.Suzuki, T. et al. (1994) EMBO J 13, 1114-22.
5.Ando, K. et al. (1999) J Neurosci 19, 4421-7.
6.Iijima, K. et al. (2000) J Neurochem 75, 1085-91.
研究领域
神经科学
翻译后修饰
unmodified
数据库链接
Entrez-Gene ID
351
UniProt ID
P05067
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