首页 抗体 一抗 一抗
beta-Catenin (D10A8) XP ® Rabbit mAb
beta-Catenin (D10A8) XP ® Rabbit mAb
1/3

beta-Catenin (D10A8) XP ® Rabbit mAb

分享
品牌： CST
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    分子量：
    92
    92
    反应种属：
    Human,Mouse,Rat,Monkey,
    Human,Mouse,Rat,Monkey,
    展开
    产品介绍
    产品信息
    抗原名称
    beta-Catenin
    来源纯化
    使用与人 β 连环蛋白 Pro714 周围残基相对应的合成肽，对动物进行免疫接种来产生单克隆抗体。
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying Catenin-beta. Cited in 1597 publications. Validated for WB, WB, IP, IHC, IHC, IF, IF, F, ChIP, ChIP, C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, β-Catenin (D10A8) XP® Rabbit Monoclonal Antibody (CST #8480) is ready to ship.
    商品描述

    Product Usage Information

    For optimal ChIP and ChIP-seq results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    ApplicationDilution
    Western Blotting1:1000
    Simple Western™1:10 - 1:50
    Immunoprecipitation1:50
    IHC Leica Bond1:50 - 1:200
    Immunohistochemistry (Paraffin)1:50 - 1:200
    Immunofluorescence (Frozen)1:50 - 1:100
    Immunofluorescence (Immunocytochemistry)1:100 - 1:400
    Flow Cytometry (Fixed/Permeabilized)1:50 - 1:100
    Chromatin IP1:25
    Chromatin IP-seq1:25
    CUT&RUN1:25
    分子量
    92
    内毒素水平
    斑马鱼 , 牛 , 猪 , 马, 豚鼠
    研究领域
    癌症,发育生物学与干细胞研究,纤维化,神经科学,
    应用
    反应种属
    Human,Mouse,Rat,Monkey,
    预测反应种属
    Zebrafish,Bovine,Pig,Horse,Guinea Pig
    目标/特异性

    Specificity/Sensitivity

    β-Catenin (D10A8) XP® Rabbit mAb 可检测总 β 连环蛋白的内源水平。

    物种反应性：

    人, 小鼠, 大鼠, 猴

    敏感性
    Endogenous
    背景
    背景
    β-联蛋白是 Wnt 信号转导通路中的一个关键下游效应子 (1)。它与脊椎动物的两个重要生物过程有关：早期胚胎发育 (2) 和肿瘤形成 (3)。CK1 可在 Ser45 位点磷酸化 β-Catenin。该磷酸化会导致 β-Catenin随后能被 GSK-3 磷酸化 (4-6)。GSK-3β 通过在 Ser33、Ser37 和 Thr41 位点磷酸化 β-Catenin以将其降解 (7)。这些位点发生突变会提高 β-Catenin的稳定性，并且已在多种肿瘤细胞系中发现了这些突变 (8)。 1.Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305. 2.Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. 3.Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. 4.Amit, S. et al. (2002) Genes Dev 16, 1066-76. 5.Liu, C. et al. (2002) Cell 108, 837-47. 6.Yanagawa, S. et al. (2002) EMBO J 21, 1733-42. 7.Yost, C. et al. (1996) Genes Dev 10, 1443-54. 8.Morin, P.J. et al. (1997) Science 275, 1787-90.
    研究领域
    癌症,发育生物学与干细胞研究,纤维化,神经科学,
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #84441.
    数据库链接
    Entrez-Gene ID
    1499
    UniProt ID
    P35222

    参考图片

    Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 (right) cells using β-Catenin (D10A8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Flow cytometric analysis of NCI-H28 (blue) or HeLa (green) cells using β-Catenin (D10A8) XP® Rabbit mAb.

    Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of frozen mouse colon using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

    Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP® Rabbit mAb.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 20 μl of β-Catenin (D10A8) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Confocal immunofluorescent analysis of mouse colon using β-Catenin (D10A8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    8480T
    一键复制
    询价
    优宁维现货
    20ul
    100ul
    抗体送U盘
    详情
    选择数量
    当前规格1件起购 
    配送至
    预计3-4个工作日送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价